Cellulose biosaccharification by Irpex lacteus wood decay fungus

Enzymatic hydrolysis is an environmentally friendly technology to produce sugars from pretreated biomass. Here, we show that the new Il-11 Irpex lacteus strain can synthesize cellulases in a high quantity. The peptone and filter paper contained medium significantly enhanced activity of endo-1,4-β-D-glucanases (app. 50 IU/mL) total 9 IU/mL), whereas with sodium carboxymethyl cellulose stimulated exo-1,4-β-D-glucanases (33 IU/mL). expression reached its maximum within 96-144 hours, optimum pH 3,7. Thermal treatment at 30 °C for 60 minutes activated cellulases, while was following 40 treatment. In total, complex (300 IU/g) saccharified untreated by 38 % 48 hours. Concentrate filter 100 IU/g more balanced enzyme-substrate ratio (2 %), which allows prolonging saccharification process will have positive effect on cost final product.